Opportunity Information: Apply for G19AS00104

The grant opportunity titled "Cooperative Ecosystem Studies Unit, Great Lakes Northern Forests CESU" (Funding Opportunity Number G19AS00104) was released by the U.S. Geological Survey (USGS), within the Department of the Interior, through the USGS Great Lakes Science Center. It is a discretionary funding opportunity offered as a cooperative agreement, which typically means the USGS expects to have substantial involvement during the project rather than simply issuing funds and stepping back. The activity falls under science and technology and other research and development, and it is listed under CFDA 15.808. The opportunity was created on July 9, 2019, had an original closing date of July 26, 2019, and anticipated making a single award with a maximum budget (award ceiling) of $70,000.

The purpose of the project is to support applied restoration science focused on cisco (Coregonus artedi), a native Great Lakes fish that has been a priority for restoration in places like Lake Huron. USGS is seeking a partner from the Cooperative Ecosystem Studies Unit (CESU) network, specifically associated with the Great Lakes Northern Forests CESU, to conduct research describing how gene expression changes during early development in cisco raised in two different settings: natural (wild) environments and hatchery environments. The underlying management need is practical and immediate: agencies are working to develop a hatchery strain that could be used to help restore cisco populations to Lake Huron, and they want to understand whether early rearing conditions in hatcheries alter gene expression in ways that could later influence adult traits (phenotype), performance, survival, or fitness once stocked.

A key theme of the opportunity is linking environment, development, and biology in a way that directly informs hatchery and fisheries management decisions. Hatchery and resource managers have long been concerned that conditions early in life, such as temperature regimes, diet, density, handling, light cycles, and other rearing practices, can shape development and lead to differences that persist into adulthood. In this project, those concerns are approached through the lens of gene expression across development, with the goal of documenting when and how expression patterns shift from hatch through metamorphosis, and how those patterns compare between hatchery-reared and wild fish.

To support the work, USGS and its agency partners have already completed important foundational steps by collecting gametes and creating experimental crosses from cisco sourced from two Great Lakes systems: Lake Huron and Lake Superior. The proposed research would use these fish and focus on comparing developmental gene expression trajectories in hatchery and wild cisco originating from northern Lake Huron and western Lake Superior. The emphasis on the period from hatch to metamorphosis reflects a biologically critical window when organ systems develop rapidly and when environmental cues can have outsized, lasting effects. The results are intended to help characterize how much the hatchery environment may be steering developmental biology away from what is observed in the wild, information that can be used to adjust rearing protocols and improve the likelihood that hatchery-produced cisco will resemble and function like wild fish when used for restoration.

Eligibility is limited to CESU partners (not broadly open to all applicants), as indicated by the "EligibleApplicants: Others" designation with clarification in the additional eligibility text field. Overall, the opportunity is a targeted, small-scale research award meant to produce actionable scientific insights about early-life gene expression in cisco, directly supporting ongoing Great Lakes restoration and hatchery strain development efforts, with a particular focus on rebuilding cisco in Lake Huron.

  • The Department of the Interior, U. S. Geological Survey in the science and technology and other research and development sector is offering a public funding opportunity titled "Cooperative Ecosystem Studies Unit, Great Lakes Northern Forests CESU" and is now available to receive applicants.
  • Interested and eligible applicants and submit their applications by referencing the CFDA number(s): 15.808.
  • This funding opportunity was created on Jul 09, 2019.
  • Applicants must submit their applications by Jul 26, 2019. (Agency may still review applications by suitable applicants for the remaining/unused allocated funding in 2026.)
  • Each selected applicant is eligible to receive up to $70,000.00 in funding.
  • The number of recipients for this funding is limited to 1 candidate(s).
  • Eligible applicants include: Others (see text field entitled Additional Information on Eligibility for clarification).
Apply for G19AS00104

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Frequently Asked Questions (FAQs)

What is the official title of this grant opportunity?

The opportunity is titled "Cooperative Ecosystem Studies Unit, Great Lakes Northern Forests CESU."

What is the Funding Opportunity Number (FON)?

The Funding Opportunity Number is G19AS00104.

Which federal agency is offering this funding?

This opportunity was released by the U.S. Geological Survey (USGS) within the Department of the Interior, through the USGS Great Lakes Science Center.

What type of award is being offered?

The funding is offered as a cooperative agreement.

What does it mean that this is a cooperative agreement?

In this opportunity, a cooperative agreement indicates that USGS expects to have substantial involvement in the project during performance, rather than simply providing funds with minimal federal participation.

Is this considered a discretionary funding opportunity?

Yes. The opportunity is described as a discretionary funding opportunity.

What is the CFDA number listed for this opportunity?

The opportunity is listed under CFDA 15.808.

What program or activity area does this opportunity fall under?

It falls under science and technology and other research and development.

When was the opportunity created?

The opportunity was created on July 9, 2019.

What was the original closing date for applications?

The original closing date was July 26, 2019.

How many awards did the agency anticipate making?

The opportunity anticipated making a single award.

What is the maximum award amount (award ceiling)?

The maximum budget (award ceiling) is $70,000.

Who is eligible to apply?

Eligibility is limited to CESU partners. The opportunity is not broadly open to all applicants and is specifically targeted to partners within the CESU network associated with the Great Lakes Northern Forests CESU.

Is this opportunity open to the general public, businesses, or organizations outside CESU?

Based on the eligibility limitation stated, it is restricted to CESU partners rather than open competition among all applicant types.

What is the main purpose of the project?

The project is intended to support applied restoration science focused on cisco (Coregonus artedi), a native Great Lakes fish prioritized for restoration, including in Lake Huron.

What scientific question is the project trying to answer?

The research aims to describe how gene expression changes during early development in cisco raised in two settings: natural (wild) environments and hatchery environments.

Why is the comparison between hatchery and wild fish important here?

Agencies are working to develop a hatchery strain to help restore cisco populations (including in Lake Huron). They want to understand whether early hatchery rearing conditions alter gene expression in ways that could influence later adult traits (phenotype), performance, survival, or fitness after stocking.

What management need is driving this research?

The practical need is to support hatchery strain development for restoration by understanding whether hatchery early-life conditions steer developmental biology away from what is observed in wild fish, and to use that information to improve rearing protocols.

Which life stage is the main focus of the study?

The emphasis is on early development from hatch through metamorphosis.

Why focus on the period from hatch to metamorphosis?

This window is described as biologically critical because organ systems develop rapidly and environmental cues during early life can have outsized and lasting effects.

What kinds of hatchery factors are mentioned as concerns for shaping development?

The opportunity notes long-standing concerns that early-life conditions such as temperature regimes, diet, density, handling, light cycles, and other rearing practices can shape development and produce differences that may persist into adulthood.

What is the key theme or approach of the project?

A central theme is linking environment, development, and biology in a way that directly informs hatchery and fisheries management decisions, using gene expression across development as the main lens for comparison.

What fish populations or source systems are included in the work described?

USGS and partners collected gametes and created experimental crosses using cisco sourced from two Great Lakes systems: Lake Huron and Lake Superior.

Which geographic areas are highlighted for the planned comparisons?

The proposed research focuses on comparing developmental gene expression trajectories in hatchery and wild cisco originating from northern Lake Huron and western Lake Superior.

What foundational work has already been completed by USGS and its partners?

The opportunity states that gametes were collected and experimental crosses were created from cisco sourced from Lake Huron and Lake Superior, providing fish for the proposed research.

What outcomes are expected from the research?

The results are intended to document when and how gene expression patterns shift across early development and how those patterns differ between hatchery-reared and wild fish, producing information that can be used to adjust hatchery rearing protocols.

How are the results intended to be used in restoration efforts?

The goal is to improve the likelihood that hatchery-produced cisco will resemble and function like wild fish when used for restoration, supporting ongoing Great Lakes restoration and hatchery strain development efforts, with particular attention to rebuilding cisco in Lake Huron.

Does the opportunity specify the award mechanism’s relationship to the CESU network?

Yes. USGS is seeking a partner from the CESU network, specifically associated with the Great Lakes Northern Forests CESU.

Is this opportunity described as large-scale or targeted?

It is described as a targeted, small-scale research award aimed at producing actionable scientific insights for near-term management needs.

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